Collectively, we discovered a new potent SIRT6 activator, which can be taken as a lead compound for later studies. ![]() You are downloading GraphPad Prism, version 9.5.1.733. ![]() Normality was confirmed by ShapiroWilk test. Visit website GraphPad Geneious Prime and Geneious Biologics are intuitive yet powerful software applications that provide DNA data analysis and visualization. We also recommend you to check the files before installation. Statistical differences in plant biomass were estimated using ordinary one-way ANOVA and corrected with Dunnett’s multiple comparison test in GraphPad Prism version 8 for Mac (GraphPad Software, Inc., San Diego, CA, USA. Graphpad Prism is industry-leading software that simplifies the analysis, graphing and presenting of scientific research. Thank you for downloading GraphPad Prism from our software library The download was scanned for viruses by our system. 21q can significantly improve the thermal stability of SIRT6 protein and inhibit the PI3K/Akt signaling pathway in mouse embryonic fibroblasts (MEFs), thereby inhibiting the proliferation of MEFs. Download GraphPad Prism If your download is not starting, click here. Molecular docking showed that the pyrazolo quinazoline of 21q formed a hydrogen bond with Val115 and four π- π interactions with Phe64, Phe82 and Phe86. The biological activity of 21q was further verified by differential scanning fluorimetry assay (DSF) and surface plasmon resonance assay (SPR). ![]() The result revealed that 2-methyl-N-(4-phenoxy-phenyl)pyrazolo quinazoline-5-amine ( 21q) was the most active compound by structure-activity relationship study, which significantly enhanced SIRT6 defatty-acylation activity with an EC 1.5 value of 1.85☐.41 μM and EC 50 value of 11.15☐.33 μM. In this study, a total of 102 pyrazolo quinazoline derivatives were designed and synthesized. SIRT6 has emerged as a novel therapeutic target for a variety of diseases.
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